BowerR64
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Man, this is a tough room for sure.
And now I will sacrifice myself and let the flaming begin on me.
What NOGE posted is good advice.
The primary reason you will not hear any (well, that much of) change in volume when pulling two of the four power valves is because the entire amp power supply is now running at a slightly higher voltage. This is why you should check the bias when doing this. The plate voltage is now higher and the bias supply voltage is also slightly higher BUT not high enough to compensate for the higher plate voltage and put the bias/idle setting back to the same setting it was at while the four power valves were in the amp. Also, the heater filament circuit is now slightly higher voltage as well. The preamp B+ will be slightly higher changing the bias of the 12AX7 preamp valves. In some amp designs, the higher preamp B+ voltage will have an influence on the tone and feel of the amp as well.
So why is everything running higher now with 2 tubes taken out? How much higher are we talking about?
Ive set my bias on 50 watt amps before really cold, the plate voltage goes up im thinking because the tube is not running as hard? Then ive set the bias hot and i noticed the plate voltage drops again i figured because its putting more load on the supply.
Does the amp consume more electricity with 2 tubes or 4 or is it the same no matter how many tubes are in it?